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H3K9me2

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K9me1
Vendor: Thermo Fisher Scientific Catalog #: PA5-11183 Lot #: TE2569662 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0293.1
0.40%
86.74
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
100.00
H3K9me1
160.44
H3K9me2
85.53
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
H3K9me2
Vendor: Thermo Fisher Scientific Catalog #: PA5-16195 Lot #: SK2484958 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0195.1
3.91%
8.38
Unmodified
6.11
H3K4me1
34.96
H3K4me2
9.37
H3K4me3
10.51
H3K9me1
100.00
H3K9me2
3.64
H3K9me3
8.90
H3K27me1
13.41
H3K27me2
7.97
H3K27me3
4.78
H3K36me1
10.66
H3K36me2
8.62
H3K36me3
3.66
H4K20me1
11.43
H4K20me2
8.67
H4K20me3
Vendor: Diagenode Catalog #: C15200154 Lot #: 1 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 8
E0316.1
4.07%
9.22
Unmodified
6.73
H3K4me1
12.24
H3K4me2
9.94
H3K4me3
16.80
H3K9me1
100.00
H3K9me2
5.38
H3K9me3
15.04
H3K27me1
10.32
H3K27me2
9.93
H3K27me3
8.24
H3K36me1
11.86
H3K36me2
9.86
H3K36me3
5.37
H4K20me1
12.14
H4K20me2
10.69
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9753 Lot #: 4 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 64
E0521.1
3.42%
9.37
Unmodified
6.13
H3K4me1
37.75
H3K4me2
9.81
H3K4me3
10.28
H3K9me1
100.00
H3K9me2
3.89
H3K9me3
8.58
H3K27me1
13.89
H3K27me2
7.85
H3K27me3
6.18
H3K36me1
12.64
H3K36me2
9.65
H3K36me3
3.64
H4K20me1
13.08
H4K20me2
9.89
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1007 Lot #: A900042 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0202.1
0.39%
24.14
Unmodified
40.08
H3K4me1
39.13
H3K4me2
15.15
H3K4me3
21.79
H3K9me1
100.00
H3K9me2
29.66
H3K9me3
41.93
H3K27me1
29.36
H3K27me2
21.74
H3K27me3
34.02
H3K36me1
30.80
H3K36me2
38.59
H3K36me3
21.93
H4K20me1
23.43
H4K20me2
22.63
H4K20me3
Vendor: EMD Millipore Catalog #: 05-1249 Lot #: 3259070 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 12
E0434.2
4.87%
15.34
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
24.33
H3K9me1
100.00
H3K9me2
8.76
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15410060 Lot #: A90-0042 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 26
E0210.1
1.54%
19.45
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
28.79
H3K9me1
100.00
H3K9me2
26.19
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-16195 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0195.2
1.07%
15.12
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
24.48
H3K9me1
100.00
H3K9me2
36.12
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720092 Lot #: RA226270 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0187.1
2.49%
24.15
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
26.40
H3K9me1
100.00
H3K9me2
29.36
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710815 Lot #: RF236633 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0085.1
0.73%
39.60
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
47.53
H3K9me1
100.00
H3K9me2
67.81
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701783 Lot #: RH239411 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0208.1
1.88%
35.38
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
32.41
H3K9me1
100.00
H3K9me2
118.21
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: EMD Millipore Catalog #: 05-1249 Lot #: 3170817 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 12
E0434.1
5.39%
10.37
Unmodified
7.55
H3K4me1
13.51
H3K4me2
10.75
H3K4me3
18.10
H3K9me1
100.00
H3K9me2
5.35
H3K9me3
15.62
H3K27me1
12.31
H3K27me2
11.08
H3K27me3
8.65
H3K36me1
11.99
H3K36me2
10.02
H3K36me3
5.10
H4K20me1
11.60
H4K20me2
10.34
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K9me2
Vendor: EMD Millipore Catalog #: 05-1249 Lot #: 3170817 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 12
E0434.1
0.50
Unmodified
0.70
H3K4me1
0.90
H3K4me2
0.60
H3K4me3
2.40
H3K9me1
100.00
H3K9me2
1.00
H3K9me3
0.90
H3K27me1
0.90
H3K27me2
0.80
H3K27me3
0.80
H3K36me1
1.20
H3K36me2
0.90
H3K36me3
1.10
H4K20me1
1.20
H4K20me2
0.80
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-16195 Lot #: SK2484958 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 2
E0195.1
0.80
Unmodified
1.20
H3K4me1
1.20
H3K4me2
0.90
H3K4me3
0.90
H3K9me1
100.00
H3K9me2
1.20
H3K9me3
0.90
H3K27me1
1.10
H3K27me2
0.90
H3K27me3
0.80
H3K36me1
1.30
H3K36me2
0.70
H3K36me3
4.70
H4K20me1
3.30
H4K20me2
3.10
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9753 Lot #: 4 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 64
E0521.1
1.00
Unmodified
1.20
H3K4me1
1.70
H3K4me2
1.40
H3K4me3
1.50
H3K9me1
100.00
H3K9me2
1.40
H3K9me3
1.30
H3K27me1
1.40
H3K27me2
1.30
H3K27me3
1.20
H3K36me1
1.90
H3K36me2
1.30
H3K36me3
5.40
H4K20me1
3.90
H4K20me2
3.70
H4K20me3
Vendor: Abcam Catalog #: ab1220 Lot #: GR3247768-1 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 677
E0419.1
1.00
Unmodified
0.90
H3K4me1
1.00
H3K4me2
0.80
H3K4me3
10.00
H3K9me1
100.00
H3K9me2
10.80
H3K9me3
1.00
H3K27me1
1.00
H3K27me2
1.20
H3K27me3
1.20
H3K36me1
1.30
H3K36me2
1.20
H3K36me3
1.00
H4K20me1
1.00
H4K20me2
1.10
H4K20me3
Vendor: Active Motif Catalog #: 39683 Lot #: 31416012 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 6
E0445.1
2.60
Unmodified
3.10
H3K4me1
4.60
H3K4me2
2.80
H3K4me3
10.20
H3K9me1
100.00
H3K9me2
3.80
H3K9me3
3.90
H3K27me1
5.00
H3K27me2
4.20
H3K27me3
2.80
H3K36me1
4.50
H3K36me2
3.30
H3K36me3
3.80
H4K20me1
6.40
H4K20me2
3.80
H4K20me3
Vendor: Active Motif Catalog #: 39240 Lot #: 28214002 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0446.1
2.00
Unmodified
2.40
H3K4me1
8.50
H3K4me2
1.90
H3K4me3
3.00
H3K9me1
100.00
H3K9me2
2.50
H3K9me3
3.00
H3K27me1
76.60
H3K27me2
2.80
H3K27me3
2.20
H3K36me1
2.90
H3K36me2
2.30
H3K36me3
2.80
H4K20me1
4.40
H4K20me2
2.60
H4K20me3
Vendor: Abcam Catalog #: ab115159 Lot #: GR3186964-6 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 6
E0421.1
2.60
Unmodified
3.90
H3K4me1
37.50
H3K4me2
4.40
H3K4me3
8.40
H3K9me1
100.00
H3K9me2
12.30
H3K9me3
4.10
H3K27me1
13.70
H3K27me2
5.20
H3K27me3
4.90
H3K36me1
5.70
H3K36me2
4.30
H3K36me3
4.40
H4K20me1
8.70
H4K20me2
5.60
H4K20me3
Vendor: Active Motif Catalog #: 39754 Lot #: 6710001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0448.1
3.70
Unmodified
4.40
H3K4me1
47.90
H3K4me2
4.50
H3K4me3
7.20
H3K9me1
100.00
H3K9me2
5.40
H3K9me3
5.40
H3K27me1
7.50
H3K27me2
5.70
H3K27me3
5.60
H3K36me1
5.20
H3K36me2
5.00
H3K36me3
5.40
H4K20me1
36.30
H4K20me2
5.70
H4K20me3
Vendor: Active Motif Catalog #: 39376 Lot #: 29108001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0447.1
3.30
Unmodified
4.20
H3K4me1
54.90
H3K4me2
4.20
H3K4me3
6.20
H3K9me1
100.00
H3K9me2
4.80
H3K9me3
4.90
H3K27me1
6.30
H3K27me2
5.00
H3K27me3
4.10
H3K36me1
5.80
H3K36me2
4.20
H3K36me3
5.30
H4K20me1
37.50
H4K20me2
5.10
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-16195 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0195.2
51.10
Unmodified
39.20
H3K4me1
3.20
H3K4me2
1.40
H3K4me3
14.00
H3K9me1
100.00
H3K9me2
15.60
H3K9me3
2.90
H3K27me1
1.90
H3K27me2
3.40
H3K27me3
2.90
H3K36me1
2.60
H3K36me2
2.70
H3K36me3
6.30
H4K20me1
5.40
H4K20me2
6.40
H4K20me3
Vendor: EMD Millipore Catalog #: 07-441 Lot #: 3030566 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 327
E0436.1
13.60
Unmodified
13.50
H3K4me1
18.80
H3K4me2
11.40
H3K4me3
18.00
H3K9me1
100.00
H3K9me2
18.70
H3K9me3
18.90
H3K27me1
19.70
H3K27me2
25.80
H3K27me3
13.00
H3K36me1
19.40
H3K36me2
14.00
H3K36me3
21.00
H4K20me1
22.40
H4K20me2
17.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710815 Lot #: RF236633 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0085.1
16.40
Unmodified
17.90
H3K4me1
35.00
H3K4me2
21.20
H3K4me3
19.40
H3K9me1
100.00
H3K9me2
34.80
H3K9me3
19.40
H3K27me1
94.10
H3K27me2
22.50
H3K27me3
16.30
H3K36me1
31.00
H3K36me2
12.20
H3K36me3
18.10
H4K20me1
18.30
H4K20me2
17.10
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1007 Lot #: A900042 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0202.1
20.50
Unmodified
27.60
H3K4me1
84.20
H3K4me2
24.80
H3K4me3
24.20
H3K9me1
100.00
H3K9me2
31.00
H3K9me3
24.40
H3K27me1
49.90
H3K27me2
24.40
H3K27me3
22.50
H3K36me1
39.70
H3K36me2
15.20
H3K36me3
25.50
H4K20me1
31.20
H4K20me2
23.40
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701783 Lot #: RH239411 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0208.1
21.50
Unmodified
21.80
H3K4me1
40.30
H3K4me2
23.10
H3K4me3
27.90
H3K9me1
100.00
H3K9me2
38.30
H3K9me3
26.90
H3K27me1
101.60
H3K27me2
27.20
H3K27me3
21.90
H3K36me1
40.60
H3K36me2
10.40
H3K36me3
27.60
H4K20me1
27.00
H4K20me2
23.80
H4K20me3
Vendor: EMD Millipore Catalog #: 07-212 Lot #: 3030285 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 158
E0435.1
18.50
Unmodified
22.60
H3K4me1
33.50
H3K4me2
20.20
H3K4me3
33.40
H3K9me1
100.00
H3K9me2
26.60
H3K9me3
30.50
H3K27me1
46.20
H3K27me2
29.10
H3K27me3
23.50
H3K36me1
35.00
H3K36me2
22.70
H3K36me3
49.00
H4K20me1
54.10
H4K20me2
40.20
H4K20me3
Vendor: Abcam Catalog #: ab176882 Lot #: GR3179038-2 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 3
E0420.1
22.40
Unmodified
23.60
H3K4me1
31.70
H3K4me2
30.90
H3K4me3
41.10
H3K9me1
100.00
H3K9me2
32.50
H3K9me3
26.60
H3K27me1
34.10
H3K27me2
35.80
H3K27me3
42.30
H3K36me1
44.70
H3K36me2
36.60
H3K36me3
41.50
H4K20me1
32.30
H4K20me2
39.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720092 Lot #: RA226270 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0187.1
55.20
Unmodified
40.20
H3K4me1
82.00
H3K4me2
24.70
H3K4me3
34.80
H3K9me1
100.00
H3K9me2
49.60
H3K9me3
31.30
H3K27me1
28.70
H3K27me2
51.40
H3K27me3
22.60
H3K36me1
40.40
H3K36me2
19.10
H3K36me3
44.90
H4K20me1
181.00
H4K20me2
56.50
H4K20me3
Vendor: EpiGentek Catalog #: A-4035-025 Lot #: 8060399 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 3
E0481.1
36.50
Unmodified
46.50
H3K4me1
67.20
H3K4me2
56.30
H3K4me3
61.10
H3K9me1
100.00
H3K9me2
58.50
H3K9me3
48.90
H3K27me1
82.70
H3K27me2
74.30
H3K27me3
40.50
H3K36me1
39.00
H3K36me2
40.00
H3K36me3
40.70
H4K20me1
54.40
H4K20me2
53.30
H4K20me3
Vendor: Abcam Catalog #: ab32521 Lot #: GR36032-12 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 11
E0479.1
36.80
Unmodified
38.00
H3K4me1
65.50
H3K4me2
30.10
H3K4me3
55.90
H3K9me1
100.00
H3K9me2
49.30
H3K9me3
57.70
H3K27me1
93.50
H3K27me2
57.90
H3K27me3
30.10
H3K36me1
64.20
H3K36me2
40.30
H3K36me3
60.90
H4K20me1
74.70
H4K20me2
50.50
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-11183 Lot #: TE2569662 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0293.1
43.20
Unmodified
43.50
H3K4me1
56.10
H3K4me2
53.90
H3K4me3
61.00
H3K9me1
100.00
H3K9me2
61.80
H3K9me3
52.30
H3K27me1
76.00
H3K27me2
43.60
H3K27me3
60.30
H3K36me1
93.20
H3K36me2
30.50
H3K36me3
64.50
H4K20me1
53.80
H4K20me2
47.40
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 4658 Lot #: 8 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 70
E0520.1
58.90
Unmodified
49.00
H3K4me1
60.50
H3K4me2
57.40
H3K4me3
61.00
H3K9me1
100.00
H3K9me2
49.00
H3K9me3
62.00
H3K27me1
61.20
H3K27me2
58.20
H3K27me3
66.90
H3K36me1
63.50
H3K36me2
80.80
H3K36me3
65.40
H4K20me1
58.60
H4K20me2
66.20
H4K20me3
Vendor: EpiGentek Catalog #: A68394-050 Lot #: 8120343 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0494.1
73.00
Unmodified
64.00
H3K4me1
90.00
H3K4me2
102.00
H3K4me3
97.00
H3K9me1
100.00
H3K9me2
95.00
H3K9me3
66.00
H3K27me1
99.00
H3K27me2
87.00
H3K27me3
123.00
H3K36me1
98.00
H3K36me2
110.00
H3K36me3
120.00
H4K20me1
76.00
H4K20me2
97.00
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3
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