Skip to Main Content

Please Make a Selection

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K36me2
Vendor: Cell Signaling Technologies Catalog #: 2901 Lot #: 5 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 30
E0524.1
6.01%
13.10
Unmodified
8.18
H3K4me1
12.66
H3K4me2
13.64
H3K4me3
13.01
H3K9me1
10.61
H3K9me2
3.13
H3K9me3
14.57
H3K27me1
10.76
H3K27me2
10.92
H3K27me3
8.81
H3K36me1
100.00
H3K36me2
13.30
H3K36me3
4.77
H4K20me1
15.78
H4K20me2
14.24
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-14867 Lot #: TC2545836 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0240.1
5.76%
14.23
Unmodified
8.79
H3K4me1
15.59
H3K4me2
15.11
H3K4me3
14.93
H3K9me1
11.43
H3K9me2
3.59
H3K9me3
14.81
H3K27me1
11.26
H3K27me2
10.70
H3K27me3
6.20
H3K36me1
100.00
H3K36me2
12.14
H3K36me3
4.62
H4K20me1
17.50
H4K20me2
14.38
H4K20me3
Vendor: Active Motif Catalog #: 61019 Lot #: 31416014 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 9
E0454.1
8.97%
15.44
Unmodified
9.17
H3K4me1
18.54
H3K4me2
16.36
H3K4me3
13.85
H3K9me1
12.39
H3K9me2
4.52
H3K9me3
15.73
H3K27me1
12.63
H3K27me2
11.61
H3K27me3
16.67
H3K36me1
100.00
H3K36me2
13.55
H3K36me3
4.57
H4K20me1
19.76
H4K20me2
14.65
H4K20me3
Vendor: Abcam Catalog #: ab176921 Lot #: GR252916-7 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0425.1
8.57%
19.11
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
27.24
H3K36me1
100.00
H3K36me2
16.68
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: EpiGentek Catalog #: A-4041-025 Lot #: 8060405 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0495.1
9.46%
19.65
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
26.39
H3K36me1
100.00
H3K36me2
41.04
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-96117 Lot #: UH2818754 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0666.1
6.36%
33.61
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
31.91
H3K36me1
100.00
H3K36me2
22.03
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: RevMAb Catalog #: 31-1047-00 Lot #: P-01-00396 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0051.1
2.15%
47.32
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
56.72
H3K36me1
100.00
H3K36me2
84.72
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15200182 Lot #: 001-12 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 1
E0366.1
0.70%
21.85
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
89.11
H3K36me1
100.00
H3K36me2
88.07
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701767 Lot #: RH239423 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0185.1
2.36%
27.85
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
106.02
H3K36me1
100.00
H3K36me2
66.58
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15310127 Lot #: A239-001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0364.1
2.79%
28.05
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
103.43
H3K36me1
100.00
H3K36me2
81.75
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: ABclonal Catalog #: A2365 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0053.1
0.36%
30.91
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
93.96
H3K36me1
100.00
H3K36me2
95.56
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Abcam Catalog #: ab9049 Lot #: GR294339-1 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 92
E0544.1
0.04%
46.64
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
86.03
H3K36me1
100.00
H3K36me2
94.12
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1039 Lot #: A239001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0207.1
3.36%
103.07
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
71.42
H3K36me1
100.00
H3K36me2
59.25
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710799 Lot #: RH239420 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0192.1
0.01%
81.86
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
57.13
H3K36me1
100.00
H3K36me2
461.31
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K36me2
Vendor: Active Motif Catalog #: 61019 Lot #: 31416014 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 9
E0454.1
0.80
Unmodified
1.20
H3K4me1
6.20
H3K4me2
1.10
H3K4me3
1.30
H3K9me1
1.60
H3K9me2
1.20
H3K9me3
1.20
H3K27me1
5.00
H3K27me2
1.20
H3K27me3
7.10
H3K36me1
100.00
H3K36me2
1.10
H3K36me3
1.20
H4K20me1
2.30
H4K20me2
1.10
H4K20me3
Vendor: Abcam Catalog #: ab176921 Lot #: GR252916-7 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 2
E0425.1
2.00
Unmodified
1.60
H3K4me1
1.70
H3K4me2
1.80
H3K4me3
3.10
H3K9me1
2.20
H3K9me2
2.70
H3K9me3
2.20
H3K27me1
2.30
H3K27me2
2.60
H3K27me3
2.80
H3K36me1
100.00
H3K36me2
2.80
H3K36me3
3.10
H4K20me1
2.10
H4K20me2
2.60
H4K20me3
Vendor: EpiGentek Catalog #: A-4041-025 Lot #: 8060405 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0495.1
2.00
Unmodified
2.30
H3K4me1
3.70
H3K4me2
2.70
H3K4me3
3.10
H3K9me1
3.00
H3K9me2
2.50
H3K9me3
2.50
H3K27me1
2.90
H3K27me2
2.90
H3K27me3
3.10
H3K36me1
100.00
H3K36me2
5.20
H3K36me3
2.60
H4K20me1
3.10
H4K20me2
2.70
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-14867 Lot #: TC2545836 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0240.1
4.20
Unmodified
4.70
H3K4me1
4.90
H3K4me2
3.80
H3K4me3
3.60
H3K9me1
3.20
H3K9me2
4.80
H3K9me3
4.10
H3K27me1
4.10
H3K27me2
4.40
H3K27me3
3.60
H3K36me1
100.00
H3K36me2
4.70
H3K36me3
3.00
H4K20me1
3.60
H4K20me2
3.60
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 2901 Lot #: 5 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 30
E0524.1
4.40
Unmodified
5.40
H3K4me1
6.10
H3K4me2
5.50
H3K4me3
5.00
H3K9me1
6.00
H3K9me2
5.50
H3K9me3
4.30
H3K27me1
5.00
H3K27me2
6.10
H3K27me3
6.30
H3K36me1
100.00
H3K36me2
6.60
H3K36me3
6.70
H4K20me1
5.20
H4K20me2
5.60
H4K20me3
Vendor: Brian Strahl Catalog #: 2 Lot #: 34655 Clonality: Species: Pass/Fail: Fail # Citations: 0
E0767.1
4.85
Unmodified
5.52
H3K4me1
7.40
H3K4me2
6.39
H3K4me3
4.82
H3K9me1
8.00
H3K9me2
7.15
H3K9me3
5.94
H3K27me1
8.32
H3K27me2
10.19
H3K27me3
5.04
H3K36me1
100.00
H3K36me2
4.36
H3K36me3
5.01
H4K20me1
6.92
H4K20me2
7.51
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-96117 Lot #: UH2818754 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0666.1
4.50
Unmodified
5.50
H3K4me1
10.30
H3K4me2
4.50
H3K4me3
4.70
H3K9me1
7.50
H3K9me2
5.00
H3K9me3
7.00
H3K27me1
8.30
H3K27me2
6.40
H3K27me3
9.70
H3K36me1
100.00
H3K36me2
7.60
H3K36me3
6.20
H4K20me1
6.20
H4K20me2
5.20
H4K20me3
Vendor: EpiGentek Catalog #: A68396 Lot #: 8120344 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0496.1
11.00
Unmodified
5.10
H3K4me1
5.80
H3K4me2
18.70
H3K4me3
5.40
H3K9me1
137.80
H3K9me2
5.30
H3K9me3
14.20
H3K27me1
9.90
H3K27me2
17.20
H3K27me3
21.80
H3K36me1
100.00
H3K36me2
20.20
H3K36me3
8.10
H4K20me1
9.60
H4K20me2
6.10
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701767 Lot #: RH239423 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0185.1
14.80
Unmodified
21.90
H3K4me1
27.60
H3K4me2
31.50
H3K4me3
16.50
H3K9me1
17.20
H3K9me2
35.60
H3K9me3
16.70
H3K27me1
21.90
H3K27me2
23.40
H3K27me3
19.40
H3K36me1
100.00
H3K36me2
22.60
H3K36me3
11.90
H4K20me1
14.00
H4K20me2
15.80
H4K20me3
Vendor: RevMAb Catalog #: 31-1047-00 Lot #: P-01-00396 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0051.1
28.87
Unmodified
28.92
H3K4me1
38.23
H3K4me2
22.43
H3K4me3
23.49
H3K9me1
60.36
H3K9me2
34.69
H3K9me3
27.24
H3K27me1
45.61
H3K27me2
27.67
H3K27me3
25.67
H3K36me1
100.00
H3K36me2
9.39
H3K36me3
36.50
H4K20me1
46.05
H4K20me2
31.14
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1039 Lot #: A239001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0207.1
44.60
Unmodified
34.30
H3K4me1
40.80
H3K4me2
20.40
H3K4me3
24.30
H3K9me1
48.10
H3K9me2
37.20
H3K9me3
28.20
H3K27me1
22.80
H3K27me2
39.00
H3K27me3
20.80
H3K36me1
100.00
H3K36me2
15.70
H3K36me3
36.90
H4K20me1
56.90
H4K20me2
41.90
H4K20me3
Vendor: Brian Strahl Catalog #: 1 Lot #: 34656 Clonality: Species: Pass/Fail: Fail # Citations: 0
E0768.1
50.50
Unmodified
40.12
H3K4me1
53.54
H3K4me2
48.18
H3K4me3
47.65
H3K9me1
89.85
H3K9me2
69.93
H3K9me3
44.45
H3K27me1
62.06
H3K27me2
85.46
H3K27me3
48.08
H3K36me1
100.00
H3K36me2
29.51
H3K36me3
48.65
H4K20me1
56.91
H4K20me2
70.52
H4K20me3
Vendor: Active Motif Catalog #: 39892 Lot #: 22313002 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0456.1
58.80
Unmodified
60.40
H3K4me1
94.30
H3K4me2
68.70
H3K4me3
73.60
H3K9me1
91.20
H3K9me2
68.30
H3K9me3
71.40
H3K27me1
78.90
H3K27me2
65.60
H3K27me3
63.40
H3K36me1
100.00
H3K36me2
72.70
H3K36me3
86.30
H4K20me1
100.20
H4K20me2
83.70
H4K20me3
Vendor: David Bentley Catalog #: 520808 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0518.1
63.60
Unmodified
74.10
H3K4me1
80.80
H3K4me2
76.60
H3K4me3
67.80
H3K9me1
99.00
H3K9me2
89.50
H3K9me3
74.30
H3K27me1
86.10
H3K27me2
104.20
H3K27me3
82.10
H3K36me1
100.00
H3K36me2
65.70
H3K36me3
65.20
H4K20me1
71.30
H4K20me2
81.80
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710799 Lot #: RH239420 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0192.1
67.50
Unmodified
111.20
H3K4me1
93.50
H3K4me2
92.30
H3K4me3
68.00
H3K9me1
80.50
H3K9me2
120.70
H3K9me3
75.70
H3K27me1
82.80
H3K27me2
104.10
H3K27me3
61.50
H3K36me1
100.00
H3K36me2
89.90
H3K36me3
62.10
H4K20me1
69.20
H4K20me2
99.40
H4K20me3
Vendor: Active Motif Catalog #: 39256 Lot #: 8308001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 3
E0455.1
67.80
Unmodified
71.60
H3K4me1
93.60
H3K4me2
78.30
H3K4me3
80.30
H3K9me1
130.60
H3K9me2
76.70
H3K9me3
96.40
H3K27me1
92.00
H3K27me2
89.00
H3K27me3
66.20
H3K36me1
100.00
H3K36me2
69.40
H3K36me3
97.10
H4K20me1
121.60
H4K20me2
84.40
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3