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H3K36me1

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K36me1
Vendor: ABclonal Catalog #: A2364 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0050.1
3.00%
4.57
Unmodified
5.17
H3K4me1
5.02
H3K4me2
4.27
H3K4me3
4.19
H3K9me1
5.17
H3K9me2
4.17
H3K9me3
9.05
H3K27me1
3.58
H3K27me2
4.64
H3K27me3
100.00
H3K36me1
6.97
H3K36me2
7.02
H3K36me3
5.86
H4K20me1
6.41
H4K20me2
4.47
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 14111 Lot #: 1 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 3
E0048.1
3.77%
7.68
Unmodified
12.77
H3K4me1
10.15
H3K4me2
5.53
H3K4me3
10.66
H3K9me1
8.69
H3K9me2
13.48
H3K9me3
25.45
H3K27me1
7.03
H3K27me2
7.38
H3K27me3
100.00
H3K36me1
11.45
H3K36me2
11.74
H3K36me3
10.38
H4K20me1
11.13
H4K20me2
7.30
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1016 Lot #: A2367001P Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0206.1
3.95%
9.97
Unmodified
14.85
H3K4me1
10.97
H3K4me2
8.64
H3K4me3
11.87
H3K9me1
10.35
H3K9me2
15.36
H3K9me3
25.89
H3K27me1
8.52
H3K27me2
9.47
H3K27me3
100.00
H3K36me1
18.69
H3K36me2
12.47
H3K36me3
10.90
H4K20me1
10.61
H4K20me2
8.58
H4K20me3
Vendor: Boster Catalog #: M12477-5 Lot #: BS191106775 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0694.1
19.71%
13.19
Unmodified
6.33
H3K4me1
12.10
H3K4me2
12.10
H3K4me3
44.86
H3K9me1
9.32
H3K9me2
5.78
H3K9me3
26.22
H3K27me1
9.85
H3K27me2
6.91
H3K27me3
100.00
H3K36me1
10.25
H3K36me2
8.43
H3K36me3
3.91
H4K20me1
11.82
H4K20me2
11.84
H4K20me3
Vendor: HuaBio Catalog #: ET1602 Lot #: HJ0122 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0693.1
8.73%
15.92
Unmodified
7.43
H3K4me1
13.29
H3K4me2
16.01
H3K4me3
32.85
H3K9me1
11.17
H3K9me2
5.68
H3K9me3
18.59
H3K27me1
12.11
H3K27me2
8.25
H3K27me3
100.00
H3K36me1
13.59
H3K36me2
10.47
H3K36me3
3.75
H4K20me1
17.78
H4K20me2
17.58
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701766 Lot #: RC226289 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0189.1
0.21%
19.90
Unmodified
25.88
H3K4me1
17.23
H3K4me2
22.38
H3K4me3
25.39
H3K9me1
31.72
H3K9me2
22.62
H3K9me3
58.63
H3K27me1
19.45
H3K27me2
22.81
H3K27me3
100.00
H3K36me1
35.66
H3K36me2
36.93
H3K36me3
45.54
H4K20me1
38.23
H4K20me2
0.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710798 Lot #: RF236632 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0190.1
0.00%
164.55
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
100.00
H3K36me1
96.30
H3K36me2
2001.29
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K36me1
Vendor: HuaBio Catalog #: ET1602 Lot #: HJ0122 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0693.1
1.10
Unmodified
1.10
H3K4me1
0.50
H3K4me2
0.50
H3K4me3
1.20
H3K9me1
0.60
H3K9me2
0.50
H3K9me3
0.90
H3K27me1
0.50
H3K27me2
0.50
H3K27me3
100.00
H3K36me1
0.70
H3K36me2
1.10
H3K36me3
0.50
H4K20me1
0.50
H4K20me2
0.50
H4K20me3
Vendor: Boster Catalog #: M12477-5 Lot #: BS191106775 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0694.1
1.50
Unmodified
1.30
H3K4me1
0.50
H3K4me2
0.50
H3K4me3
1.00
H3K9me1
0.90
H3K9me2
0.60
H3K9me3
0.70
H3K27me1
0.50
H3K27me2
0.50
H3K27me3
100.00
H3K36me1
0.60
H3K36me2
1.10
H3K36me3
0.50
H4K20me1
0.40
H4K20me2
0.40
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 14111 Lot #: 1 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 3
E0048.1
0.30
Unmodified
1.30
H3K4me1
0.40
H3K4me2
0.30
H3K4me3
1.50
H3K9me1
0.50
H3K9me2
0.40
H3K9me3
7.40
H3K27me1
0.30
H3K27me2
0.40
H3K27me3
100.00
H3K36me1
0.40
H3K36me2
0.40
H3K36me3
1.60
H4K20me1
0.30
H4K20me2
0.30
H4K20me3
Vendor: ABclonal Catalog #: A2364 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0050.2
1.10
Unmodified
6.00
H3K4me1
1.40
H3K4me2
1.00
H3K4me3
2.20
H3K9me1
2.30
H3K9me2
1.30
H3K9me3
2.00
H3K27me1
1.00
H3K27me2
1.30
H3K27me3
100.00
H3K36me1
2.40
H3K36me2
0.90
H3K36me3
1.40
H4K20me1
1.10
H4K20me2
1.40
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-96116 Lot #: UG2800236 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0641.1
0.30
Unmodified
18.60
H3K4me1
0.50
H3K4me2
0.40
H3K4me3
10.10
H3K9me1
0.40
H3K9me2
0.40
H3K9me3
14.20
H3K27me1
0.50
H3K27me2
0.60
H3K27me3
100.00
H3K36me1
0.70
H3K36me2
3.00
H3K36me3
4.40
H4K20me1
0.50
H4K20me2
0.40
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1016 Lot #: A2367001P Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0206.1
2.90
Unmodified
7.30
H3K4me1
3.00
H3K4me2
3.40
H3K4me3
6.60
H3K9me1
4.50
H3K9me2
3.90
H3K9me3
8.40
H3K27me1
4.00
H3K27me2
3.30
H3K27me3
100.00
H3K36me1
12.30
H3K36me2
2.40
H3K36me3
6.60
H4K20me1
3.20
H4K20me2
3.10
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710798 Lot #: RF236632 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0190.1
99.30
Unmodified
153.30
H3K4me1
95.30
H3K4me2
106.00
H3K4me3
96.00
H3K9me1
122.70
H3K9me2
136.00
H3K9me3
92.70
H3K27me1
96.00
H3K27me2
123.30
H3K27me3
100.00
H3K36me1
130.00
H3K36me2
106.70
H3K36me3
76.00
H4K20me1
73.30
H4K20me2
121.30
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701766 Lot #: RC226289 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0189.1
56.20
Unmodified
204.50
H3K4me1
96.90
H3K4me2
122.60
H3K4me3
227.80
H3K9me1
52.00
H3K9me2
146.30
H3K9me3
245.70
H3K27me1
78.20
H3K27me2
88.40
H3K27me3
100.00
H3K36me1
75.60
H3K36me2
100.00
H3K36me3
267.00
H4K20me1
47.90
H4K20me2
52.80
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3
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