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For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H3K23ac
Vendor: EMD Millipore Catalog #: 07-355 Lot #: 3188630 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 44
E0318.1
8.89%
3.57
Unmodified
4.49
H3K4ac
2.72
H3K9ac
4.89
H3K9bu
4.44
H3K9cr
4.84
H3K14ac
3.44
H3K18ac
3.38
H3K18bu
4.44
H3K18cr
15.41
tetraAc-H3
100.00
H3K23ac
4.31
H3K27ac
5.07
H3K27bu
3.19
H3K27cr
6.42
H3K27acS28ph
6.21
H3K36ac
8.87
H4K5ac
2.11
H4K8ac
3.95
H4K12ac
4.50
H4K16ac
3.93
tetraAc-H4
5.91
H4K20ac
8.06
tetraAc-H2A
Vendor: Abcam Catalog #: ab177275 Lot #: GR3213108-2 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0323.1
1.96%
14.68
Unmodified
25.61
H3K4ac
18.35
H3K9ac
15.68
H3K9bu
16.52
H3K9cr
38.47
H3K14ac
16.55
H3K18ac
13.10
H3K18bu
17.97
H3K18cr
769.10
tetraAc-H3
100.00
H3K23ac
19.98
H3K27ac
19.55
H3K27bu
13.31
H3K27cr
54.30
H3K27acS28ph
30.82
H3K36ac
57.97
H4K5ac
12.99
H4K8ac
28.53
H4K12ac
19.99
H4K16ac
166.11
tetraAc-H4
36.47
H4K20ac
32.22
tetraAc-H2A
Vendor: Abcam Catalog #: ab177275 Lot #: GR3213108-3 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0323.2
2.13%
11.77
Unmodified
11.52
H3K4ac
12.11
H3K9ac
9.90
H3K9bu
110.94
H3K9cr
13.23
H3K14ac
8.18
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
117.13
tetraAc-H3
100.00
H3K23ac
7.10
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
6.62
H4K8ac
No Data
H4K12ac
9.90
H4K16ac
17.08
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1087-00 Lot #: P-10-00722 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0026.1
0.95%
122.87
Unmodified
70.06
H3K4ac
8.86
H3K9ac
4.97
H3K9bu
No Data
H3K9cr
No Data
H3K14ac
No Data
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
218.98
tetraAc-H3
100.00
H3K23ac
29.96
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
No Data
H4K8ac
No Data
H4K12ac
No Data
H4K16ac
No Data
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Efficiency
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H3K23ac
Vendor: EMD Millipore Catalog #: 07-355 Lot #: 3188630 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 44
E0318.1
0.30
Unmodified
0.40
H3K4ac
2.80
H3K9ac
0.30
H3K9bu
0.40
H3K9cr
0.60
H3K14ac
0.60
H3K18ac
0.30
H3K18bu
0.30
H3K18cr
3.20
tetraAc-H3
100.00
H3K23ac
0.90
H3K27ac
0.30
H3K27bu
0.30
H3K27cr
0.30
H3K27acS28ph
0.20
H3K36ac
0.50
H4K5ac
0.40
H4K8ac
0.60
H4K12ac
0.60
H4K16ac
2.20
tetraAc-H4
1.00
H4K20ac
9.10
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1087-00 Lot #: R-05-01547 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0026.2
68.60
Unmodified
57.10
H3K4ac
89.10
H3K9ac
64.00
H3K9bu
86.90
H3K9cr
163.40
H3K14ac
60.60
H3K18ac
76.60
H3K18bu
53.10
H3K18cr
88.00
tetraAc-H3
100.00
H3K23ac
86.90
H3K27ac
73.70
H3K27bu
84.60
H3K27cr
64.00
H3K27acS28ph
84.60
H3K36ac
84.60
H4K5ac
81.10
H4K8ac
80.00
H4K12ac
79.40
H4K16ac
107.40
tetraAc-H4
86.90
H4K20ac
78.30
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1087-00 Lot #: P-10-00722 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0026.1
95.70
Unmodified
76.10
H3K4ac
114.10
H3K9ac
95.70
H3K9bu
113.00
H3K9cr
178.30
H3K14ac
88.00
H3K18ac
104.30
H3K18bu
75.00
H3K18cr
158.70
tetraAc-H3
100.00
H3K23ac
90.20
H3K27ac
89.10
H3K27bu
91.30
H3K27cr
105.40
H3K27acS28ph
106.50
H3K36ac
102.20
H4K5ac
110.90
H4K8ac
101.10
H4K12ac
100.00
H4K16ac
119.60
tetraAc-H4
117.40
H4K20ac
117.40
tetraAc-H2A
Vendor: Abcam Catalog #: ab177275 Lot #: GR3213108-2 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0323.1
56.30
Unmodified
66.70
H3K4ac
76.40
H3K9ac
80.60
H3K9bu
79.20
H3K9cr
114.60
H3K14ac
84.70
H3K18ac
81.90
H3K18bu
75.70
H3K18cr
127.80
tetraAc-H3
100.00
H3K23ac
98.60
H3K27ac
70.80
H3K27bu
57.60
H3K27cr
73.60
H3K27acS28ph
85.40
H3K36ac
115.30
H4K5ac
377.80
H4K8ac
101.40
H4K12ac
84.70
H4K16ac
250.00
tetraAc-H4
110.40
H4K20ac
177.80
tetraAc-H2A
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A