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H4K8

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H4K8ac
Vendor: Thermo Fisher Scientific Catalog #: 701796 Lot #: 2123329 Clonality: Recombinant Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0659.1
78.14%
0.22
Unmodified
0.28
H3K4ac
0.15
H3K9ac
0.34
H3K9bu
0.44
H3K9cr
0.30
H3K14ac
0.30
H3K18ac
0.22
H3K18bu
0.39
H3K18cr
0.19
tetraAc-H3
0.25
H3K23ac
0.20
H3K27ac
0.31
H3K27bu
0.20
H3K27cr
0.54
H3K27acS28ph
3.52
H3K36ac
0.19
H4K5ac
100.00
H4K8ac
0.20
H4K12ac
0.23
H4K16ac
0.24
tetraAc-H4
0.23
H4K20ac
0.58
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: 710828 Lot #: 2021259 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0648.1
54.50%
0.28
Unmodified
0.38
H3K4ac
0.20
H3K9ac
0.40
H3K9bu
0.51
H3K9cr
0.33
H3K14ac
0.36
H3K18ac
0.32
H3K18bu
0.37
H3K18cr
0.27
tetraAc-H3
0.22
H3K23ac
0.25
H3K27ac
0.41
H3K27bu
0.09
H3K27cr
0.63
H3K27acS28ph
5.06
H3K36ac
0.22
H4K5ac
100.00
H4K8ac
0.17
H4K12ac
0.22
H4K16ac
0.21
tetraAc-H4
0.07
H4K20ac
0.92
tetraAc-H2A
Vendor: EpiCypher Catalog #: 13-0036 Lot #: 19091001 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0781.1
51.73%
0.37
Unmodified
0.76
H3K4ac
0.34
H3K9ac
0.72
H3K9bu
0.44
H3K9cr
0.58
H3K14ac
0.47
H3K18ac
0.44
H3K18bu
0.52
H3K18cr
7.07
tetraAc-H3
0.56
H3K23ac
0.60
H3K27ac
0.56
H3K27bu
0.33
H3K27cr
1.02
H3K27acS28ph
0.85
H3K36ac
0.94
H4K5ac
100.00
H4K8ac
0.74
H4K12ac
0.64
H4K16ac
12.19
tetraAc-H4
0.76
H4K20ac
1.41
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1082-00 Lot #: S-07-02389 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0502.2
84.38%
0.57
Unmodified
0.79
H3K4ac
0.55
H3K9ac
0.86
H3K9bu
0.78
H3K9cr
1.02
H3K14ac
0.64
H3K18ac
0.66
H3K18bu
0.81
H3K18cr
9.24
tetraAc-H3
0.98
H3K23ac
0.77
H3K27ac
0.82
H3K27bu
0.46
H3K27cr
1.97
H3K27acS28ph
1.96
H3K36ac
1.35
H4K5ac
100.00
H4K8ac
1.43
H4K12ac
0.72
H4K16ac
17.72
tetraAc-H4
0.74
H4K20ac
1.48
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: MA5-33386 Lot #: VB2942399 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0846.1
84.38%
0.57
Unmodified
0.79
H3K4ac
0.55
H3K9ac
0.86
H3K9bu
0.78
H3K9cr
1.02
H3K14ac
0.64
H3K18ac
0.66
H3K18bu
0.81
H3K18cr
9.24
tetraAc-H3
0.98
H3K23ac
0.77
H3K27ac
0.82
H3K27bu
0.46
H3K27cr
1.97
H3K27acS28ph
1.96
H3K36ac
1.35
H4K5ac
100.00
H4K8ac
1.43
H4K12ac
0.72
H4K16ac
17.72
tetraAc-H4
0.74
H4K20ac
1.48
tetraAc-H2A
Efficiency
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H4K8ac
Vendor: Thermo Fisher Scientific Catalog #: 701796 Lot #: 2123329 Clonality: Recombinant Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0659.1
0.00
Unmodified
0.10
H3K4ac
0.10
H3K9ac
0.00
H3K9bu
0.00
H3K9cr
0.00
H3K14ac
0.10
H3K18ac
0.10
H3K18bu
0.00
H3K18cr
0.10
tetraAc-H3
0.10
H3K23ac
0.00
H3K27ac
0.10
H3K27bu
0.00
H3K27cr
0.00
H3K27acS28ph
0.00
H3K36ac
0.00
H4K5ac
100.00
H4K8ac
0.00
H4K12ac
0.10
H4K16ac
0.00
tetraAc-H4
0.10
H4K20ac
0.00
tetraAc-H2A
Vendor: EpiCypher Catalog #: 13-1021 Lot #: 15225001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0308.1
0.20
Unmodified
0.20
H3K4ac
0.10
H3K9ac
0.10
H3K9bu
0.20
H3K9cr
0.20
H3K14ac
0.10
H3K18ac
0.10
H3K18bu
0.10
H3K18cr
0.10
tetraAc-H3
0.10
H3K23ac
0.20
H3K27ac
0.10
H3K27bu
0.10
H3K27cr
0.20
H3K27acS28ph
0.20
H3K36ac
0.20
H4K5ac
100.00
H4K8ac
0.20
H4K12ac
0.20
H4K16ac
72.00
tetraAc-H4
0.20
H4K20ac
0.30
tetraAc-H2A
Vendor: ABclonal Catalog #: A7258 Lot #: 2300080401 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 3
E0179.1
0.10
Unmodified
0.10
H3K4ac
0.10
H3K9ac
0.10
H3K9bu
0.10
H3K9cr
0.10
H3K14ac
0.10
H3K18ac
0.10
H3K18bu
0.10
H3K18cr
0.10
tetraAc-H3
0.10
H3K23ac
0.10
H3K27ac
0.10
H3K27bu
0.10
H3K27cr
0.10
H3K27acS28ph
0.10
H3K36ac
0.20
H4K5ac
100.00
H4K8ac
1.80
H4K12ac
0.10
H4K16ac
30.00
tetraAc-H4
0.10
H4K20ac
0.40
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: 710828 Lot #: 2021259 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0648.1
0.30
Unmodified
0.20
H3K4ac
0.30
H3K9ac
0.30
H3K9bu
0.40
H3K9cr
0.40
H3K14ac
0.20
H3K18ac
0.20
H3K18bu
0.30
H3K18cr
0.30
tetraAc-H3
0.20
H3K23ac
0.40
H3K27ac
0.30
H3K27bu
0.30
H3K27cr
0.30
H3K27acS28ph
0.20
H3K36ac
0.30
H4K5ac
100.00
H4K8ac
0.30
H4K12ac
0.30
H4K16ac
0.40
tetraAc-H4
0.40
H4K20ac
0.60
tetraAc-H2A
Vendor: EpiCypher Catalog #: 13-0036 Lot #: 19091001 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0781.1
0.20
Unmodified
0.40
H3K4ac
0.40
H3K9ac
0.40
H3K9bu
0.40
H3K9cr
0.50
H3K14ac
0.40
H3K18ac
0.50
H3K18bu
0.40
H3K18cr
0.80
tetraAc-H3
0.50
H3K23ac
0.50
H3K27ac
0.60
H3K27bu
0.40
H3K27cr
1.40
H3K27acS28ph
0.40
H3K36ac
0.90
H4K5ac
100.00
H4K8ac
1.60
H4K12ac
0.70
H4K16ac
23.20
tetraAc-H4
0.60
H4K20ac
1.30
tetraAc-H2A
Vendor: ABclonal Catalog #: A7258 Lot #: 2300080301 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 3
E0179.2
0.60
Unmodified
0.60
H3K4ac
0.60
H3K9ac
0.60
H3K9bu
0.70
H3K9cr
0.70
H3K14ac
0.70
H3K18ac
0.70
H3K18bu
0.70
H3K18cr
0.90
tetraAc-H3
0.60
H3K23ac
0.60
H3K27ac
0.60
H3K27bu
0.50
H3K27cr
0.60
H3K27acS28ph
0.60
H3K36ac
4.40
H4K5ac
100.00
H4K8ac
2.50
H4K12ac
0.60
H4K16ac
75.00
tetraAc-H4
0.50
H4K20ac
1.80
tetraAc-H2A
Vendor: Abcam Catalog #: ab45166 Lot #: GR3238898-1 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 8
E0556.1
0.20
Unmodified
0.20
H3K4ac
0.30
H3K9ac
0.20
H3K9bu
0.30
H3K9cr
0.40
H3K14ac
4.00
H3K18ac
0.80
H3K18bu
0.30
H3K18cr
3.70
tetraAc-H3
0.20
H3K23ac
0.30
H3K27ac
0.30
H3K27bu
0.20
H3K27cr
0.30
H3K27acS28ph
0.20
H3K36ac
0.80
H4K5ac
100.00
H4K8ac
0.90
H4K12ac
0.30
H4K16ac
74.80
tetraAc-H4
15.50
H4K20ac
0.90
tetraAc-H2A
Vendor: ABclonal Catalog #: A7258 Lot #: 2300080101 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 3
E0179.3
2.90
Unmodified
3.60
H3K4ac
3.60
H3K9ac
3.30
H3K9bu
3.90
H3K9cr
3.60
H3K14ac
3.50
H3K18ac
3.50
H3K18bu
3.60
H3K18cr
3.60
tetraAc-H3
3.40
H3K23ac
3.50
H3K27ac
2.90
H3K27bu
3.10
H3K27cr
2.80
H3K27acS28ph
3.00
H3K36ac
0.50
H4K5ac
100.00
H4K8ac
0.70
H4K12ac
0.70
H4K16ac
6.40
tetraAc-H4
0.70
H4K20ac
3.10
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1082-00 Lot #: S-07-02389 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0502.2
4.60
Unmodified
3.30
H3K4ac
3.30
H3K9ac
4.80
H3K9bu
5.90
H3K9cr
6.30
H3K14ac
3.70
H3K18ac
3.70
H3K18bu
4.20
H3K18cr
9.70
tetraAc-H3
4.40
H3K23ac
6.90
H3K27ac
5.50
H3K27bu
6.00
H3K27cr
15.70
H3K27acS28ph
3.20
H3K36ac
9.70
H4K5ac
100.00
H4K8ac
12.80
H4K12ac
5.30
H4K16ac
91.70
tetraAc-H4
6.00
H4K20ac
15.60
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: 720105 Lot #: 2123012 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0658.1
9.30
Unmodified
5.80
H3K4ac
7.20
H3K9ac
9.80
H3K9bu
11.20
H3K9cr
13.40
H3K14ac
6.60
H3K18ac
7.60
H3K18bu
8.30
H3K18cr
12.80
tetraAc-H3
8.40
H3K23ac
14.30
H3K27ac
10.80
H3K27bu
12.40
H3K27cr
17.60
H3K27acS28ph
6.30
H3K36ac
15.80
H4K5ac
100.00
H4K8ac
21.10
H4K12ac
8.10
H4K16ac
161.80
tetraAc-H4
12.40
H4K20ac
37.50
tetraAc-H2A
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A
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