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For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H3K14ac
Vendor: Abcam Catalog #: ab52946 Lot #: GR3252548-3 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 65
E0321.1
20.72%
4.94
Unmodified
3.16
H3K4ac
4.46
H3K9ac
5.10
H3K9bu
4.70
H3K9cr
100.00
H3K14ac
2.47
H3K18ac
2.84
H3K18bu
3.42
H3K18cr
251.42
tetraAc-H3
3.01
H3K23ac
3.21
H3K27ac
3.86
H3K27bu
2.59
H3K27cr
4.52
H3K27acS28ph
3.96
H3K36ac
0.00
H4K5ac
1.40
H4K8ac
3.25
H4K12ac
3.16
H4K16ac
2.74
tetraAc-H4
3.65
H4K20ac
8.21
tetraAc-H2A
Vendor: Abcam Catalog #: ab52946 Lot #: GR149741-20 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 65
E0321.2
28.44%
3.11
Unmodified
3.57
H3K4ac
2.45
H3K9ac
3.53
H3K9bu
3.31
H3K9cr
100.00
H3K14ac
2.95
H3K18ac
3.03
H3K18bu
3.53
H3K18cr
210.52
tetraAc-H3
4.00
H3K23ac
3.96
H3K27ac
4.63
H3K27bu
2.85
H3K27cr
6.89
H3K27acS28ph
4.42
H3K36ac
5.31
H4K5ac
2.18
H4K8ac
4.32
H4K12ac
4.02
H4K16ac
3.72
tetraAc-H4
6.69
H4K20ac
7.33
tetraAc-H2A
Vendor: Cell Signaling Technologies Catalog #: 7627 Lot #: 5 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 30
E0476.1
2.30%
5.96
Unmodified
6.81
H3K4ac
3.97
H3K9ac
7.03
H3K9bu
8.82
H3K9cr
100.00
H3K14ac
5.62
H3K18ac
5.54
H3K18bu
9.55
H3K18cr
740.01
tetraAc-H3
5.42
H3K23ac
5.38
H3K27ac
7.97
H3K27bu
5.33
H3K27cr
14.88
H3K27acS28ph
14.59
H3K36ac
26.19
H4K5ac
2.53
H4K8ac
7.89
H4K12ac
7.26
H4K16ac
4.54
tetraAc-H4
8.52
H4K20ac
16.26
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: MA5-24668 Lot #: N/I Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0017.1
0.19%
24.13
Unmodified
193.18
H3K4ac
179.37
H3K9ac
80.77
H3K9bu
No Data
H3K9cr
100.00
H3K14ac
49.36
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
1209.34
tetraAc-H3
No Data
H3K23ac
23.29
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
No Data
H4K8ac
No Data
H4K12ac
No Data
H4K16ac
No Data
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Vendor: EMD Millipore Catalog #: 07-353 Lot #: 2816727 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 212
E0013.1
1.51%
6.47
Unmodified
14.69
H3K4ac
123.69
H3K9ac
11.31
H3K9bu
No Data
H3K9cr
100.00
H3K14ac
11.99
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
196.54
tetraAc-H3
No Data
H3K23ac
6.71
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
13.78
H4K8ac
No Data
H4K12ac
No Data
H4K16ac
9.38
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Vendor: ABclonal Catalog #: A7254 Lot #: 2300020501 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 3
E0182.1
0.43%
15.06
Unmodified
No Data
H3K4ac
516.59
H3K9ac
No Data
H3K9bu
No Data
H3K9cr
100.00
H3K14ac
30.66
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
192.13
tetraAc-H3
No Data
H3K23ac
14.78
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
49.64
H4K8ac
No Data
H4K12ac
No Data
H4K16ac
20.83
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1032-00 Lot #: P-01-00405 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0014.1
0.52%
123.30
Unmodified
No Data
H3K4ac
341.94
H3K9ac
No Data
H3K9bu
No Data
H3K9cr
100.00
H3K14ac
77.58
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
161.39
tetraAc-H3
No Data
H3K23ac
No Data
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
42.03
H4K8ac
No Data
H4K12ac
40.26
H4K16ac
186.94
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Efficiency
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from orange (weak activity) to dark blue (strong activity). Increasing blue across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H3K14ac
Vendor: Abcam Catalog #: ab52946 Lot #: GR3252548-3 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 65
E0321.1
0.20
Unmodified
0.10
H3K4ac
0.20
H3K9ac
0.20
H3K9bu
0.20
H3K9cr
100.00
H3K14ac

H3K18ac
0.20
H3K18bu
0.20
H3K18cr
116.20
tetraAc-H3
0.20
H3K23ac
0.20
H3K27ac
0.20
H3K27bu
0.20
H3K27cr
0.20
H3K27acS28ph
0.20
H3K36ac
0.20
H4K5ac
0.20
H4K8ac
0.20
H4K12ac
0.20
H4K16ac
0.30
tetraAc-H4
0.30
H4K20ac
6.90
tetraAc-H2A
Vendor: Active Motif Catalog #: 61433 Lot #: 10715003 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 1
E0558.1
4.50
Unmodified
5.00
H3K4ac
6.30
H3K9ac
6.20
H3K9bu
4.50
H3K9cr
100.00
H3K14ac
4.50
H3K18ac
5.90
H3K18bu
5.00
H3K18cr
293.80
tetraAc-H3
4.80
H3K23ac
5.60
H3K27ac
5.00
H3K27bu
7.30
H3K27cr
5.50
H3K27acS28ph
5.30
H3K36ac
5.40
H4K5ac
6.10
H4K8ac
5.00
H4K12ac
5.10
H4K16ac
6.80
tetraAc-H4
5.90
H4K20ac
7.00
tetraAc-H2A
Vendor: Abcam Catalog #: ab52946 Lot #: GR149741-20 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 65
E0321.2
2.50
Unmodified
3.50
H3K4ac
3.90
H3K9ac
3.50
H3K9bu
4.00
H3K9cr
100.00
H3K14ac
3.40
H3K18ac
3.60
H3K18bu
3.10
H3K18cr
93.40
tetraAc-H3
3.50
H3K23ac
3.80
H3K27ac
3.70
H3K27bu
4.40
H3K27cr
4.60
H3K27acS28ph
4.20
H3K36ac
3.60
H4K5ac
3.20
H4K8ac
3.40
H4K12ac
3.90
H4K16ac
4.20
tetraAc-H4
4.30
H4K20ac
4.60
tetraAc-H2A
Vendor: Cell Signaling Technologies Catalog #: 7627 Lot #: 5 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 30
E0476.1
8.10
Unmodified
6.10
H3K4ac
9.90
H3K9ac
8.70
H3K9bu
9.80
H3K9cr
100.00
H3K14ac
7.60
H3K18ac
8.00
H3K18bu
8.40
H3K18cr
1527.50
tetraAc-H3
7.40
H3K23ac
9.90
H3K27ac
7.40
H3K27bu
8.70
H3K27cr
10.30
H3K27acS28ph
9.60
H3K36ac
8.70
H4K5ac
9.10
H4K8ac
7.00
H4K12ac
6.50
H4K16ac
8.20
tetraAc-H4
10.50
H4K20ac
12.60
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: MA5-24668 Lot #: N/I Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0017.1
4.60
Unmodified
4.00
H3K4ac
5.60
H3K9ac
4.60
H3K9bu
7.00
H3K9cr
100.00
H3K14ac
4.20
H3K18ac
4.00
H3K18bu
4.00
H3K18cr
863.00
tetraAc-H3
5.80
H3K23ac
6.20
H3K27ac
3.50
H3K27bu
4.30
H3K27cr
5.30
H3K27acS28ph
5.80
H3K36ac
6.50
H4K5ac
4.90
H4K8ac
4.30
H4K12ac
4.30
H4K16ac
21.70
tetraAc-H4
5.30
H4K20ac
7.40
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1032-00 Lot #: P-01-00405 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0014.1
3.90
Unmodified
3.20
H3K4ac
5.10
H3K9ac
5.50
H3K9bu
5.50
H3K9cr
100.00
H3K14ac
5.60
H3K18ac
4.30
H3K18bu
4.30
H3K18cr
895.40
tetraAc-H3
4.00
H3K23ac
6.70
H3K27ac
5.20
H3K27bu
6.30
H3K27cr
5.00
H3K27acS28ph
5.80
H3K36ac
7.00
H4K5ac
6.50
H4K8ac
5.50
H4K12ac
5.80
H4K16ac
28.80
tetraAc-H4
5.50
H4K20ac
7.20
tetraAc-H2A
Vendor: EMD Millipore Catalog #: 07-353 Lot #: 2816727 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 212
E0013.1
1.10
Unmodified
3.20
H3K4ac
1.00
H3K9ac
0.80
H3K9bu
0.90
H3K9cr
100.00
H3K14ac
1.20
H3K18ac
1.60
H3K18bu
1.10
H3K18cr
140.30
tetraAc-H3
1.00
H3K23ac
1.10
H3K27ac
1.00
H3K27bu
1.10
H3K27cr
1.20
H3K27acS28ph
1.10
H3K36ac
1.50
H4K5ac
2.50
H4K8ac
3.50
H4K12ac
2.10
H4K16ac
8.50
tetraAc-H4
1.70
H4K20ac
17.30
tetraAc-H2A
Vendor: EMD Millipore Catalog #: 07-353 Lot #: 3011823A Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 212
E0013.2
1.10
Unmodified
2.40
H3K4ac
1.10
H3K9ac
0.90
H3K9bu
1.00
H3K9cr
100.00
H3K14ac
1.20
H3K18ac
1.40
H3K18bu
1.20
H3K18cr
162.80
tetraAc-H3
1.40
H3K23ac
1.30
H3K27ac
1.20
H3K27bu
1.20
H3K27cr
1.30
H3K27acS28ph
1.20
H3K36ac
1.50
H4K5ac
2.50
H4K8ac
3.90
H4K12ac
2.00
H4K16ac
11.10
tetraAc-H4
1.80
H4K20ac
19.00
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: 720094 Lot #: RC230347 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0707.1
30.10
Unmodified
31.70
H3K4ac
34.00
H3K9ac
29.50
H3K9bu
35.60
H3K9cr
100.00
H3K14ac
34.80
H3K18ac
35.00
H3K18bu
33.60
H3K18cr
986.50
tetraAc-H3
37.60
H3K23ac
43.40
H3K27ac
38.90
H3K27bu
44.00
H3K27cr
47.10
H3K27acS28ph
28.40
H3K36ac
40.50
H4K5ac
37.20
H4K8ac
48.20
H4K12ac
37.70
H4K16ac
81.30
tetraAc-H4
55.20
H4K20ac
87.30
tetraAc-H2A
Vendor: EpiGentek Catalog #: C10010-1 Lot #: 3K14A Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0487.1
12.80
Unmodified
11.80
H3K4ac
16.70
H3K9ac
12.50
H3K9bu
19.00
H3K9cr
100.00
H3K14ac
49.70
H3K18ac
26.10
H3K18bu
23.80
H3K18cr
108.60
tetraAc-H3
9.90
H3K23ac
27.20
H3K27ac
14.10
H3K27bu
17.70
H3K27cr
21.70
H3K27acS28ph
12.00
H3K36ac
23.10
H4K5ac
79.30
H4K8ac
15.80
H4K12ac
13.90
H4K16ac
62.10
tetraAc-H4
19.60
H4K20ac
46.50
tetraAc-H2A
Vendor: ABclonal Catalog #: A7254 Lot #: 2300020501 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 3
E0182.1
2.20
Unmodified
2.50
H3K4ac
2.70
H3K9ac
1.90
H3K9bu
2.90
H3K9cr
100.00
H3K14ac
2.30
H3K18ac
3.80
H3K18bu
2.90
H3K18cr
66.20
tetraAc-H3
3.00
H3K23ac
4.50
H3K27ac
3.50
H3K27bu
2.20
H3K27cr
4.20
H3K27acS28ph
2.80
H3K36ac
3.90
H4K5ac
38.00
H4K8ac
6.50
H4K12ac
3.90
H4K16ac
23.40
tetraAc-H4
5.60
H4K20ac
679.80
tetraAc-H2A
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A